Hamlet

Where does Gertrude finally realize Claudius' treachery?

Act 5 Scene 2

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In Scene V, Act II, when Gertrude toasts to Hamlet’s health, she takes up the poisoned chalice and has a drink despite Claudius’ protestations. Minutes later, the queen collapses and declares that she has been poisoned by the drink and then dies.

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http://www.gradesaver.com/hamlet/study-guide/summary-act-5