consider the ways in which the setting can be significant to the overall themes in work of literature?

The setting of the story is WWII Amersterdam. The underlying theme is of course the treatment and anihilation of the Jews. This novel is a non-fiction narrative; I'm not sure what you mean about how it contributes? This was a real place, a real war, and a real teenage girl. Anne's story represents millions of teenage Jewish girls and their families during Hitler's regime.