Microbiology: An Introduction, 11th Edition

Published by Benjamin Cummings
ISBN 10: 0321733606
ISBN 13: 978-0-32173-360-3

Chapter 3 - Figure 3.3 - Refraction in the compound microscope using an oil immersion objective lens - Question - Page 59: 1

Answer

It is necessary to keep the light rays from refracting.

Work Step by Step

While this is not a problem at lower levels, and magnifications over 900 times, it is necessary to consider how the light rays will refract. By adding oil, which has the same refractive index as glass, this problem is solved.
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