Answer
When preparing slides for electron microscope, the objects are placed between the slides and hence air is removed. The objects require oxygen and in the absence of oxygen, they die.
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Microbiology with Diseases by Body System (4th Edition)
by Bauman, Robert W,, PhD
Published by
Benjamin Cummings
ISBN 10:
032191855X
ISBN 13:
978-0-32191-855-0
Chapter 4 - Microscopy, Staining, and Classification - Questions For Review - Short Answer - Page 123: 3
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