Answer
The AN-PEP enzymes from mold and its analogous enzymes that do not digest the protein-rich peptide would most likely differ in the primary sequence of the polypeptide that would contribute to forming the active site of the enzyme.
Work Step by Step
The primary sequence of a polypeptide directs the functional three-dimensional tertiary structure of a protein.
AN-PEP’s ability to digest proline-rich peptides likely stems from unique structural adaptations in its active site, including flexibility, shape, and residue composition that allow it to overcome the challenges posed by proline’s rigid structure. These features distinguish it from enzymes that fail to process gluten fragments effectively.