Answer
If the pulse-chase experiment were to be conducted using muscle cells, the labeled proteins would be actin and myosin and they would be observed in the cytosol and mitochondria.
Work Step by Step
The pulse-chase experiment tracks the movement of proteins through the endomembrane system. In this experiment, a set of molecules are radiolabeled at a specific time, which is called the pulse, and their direction is followed with respect to time, which is called the chase.
Actin and myosin filaments would end up in the cytosol and mitochondria because they are cytosolic proteins required for the contraction of muscles. Muscle contraction requires a high amount of energy, which is produced by mitochondria in the form of ATP.