Answer
Class I aldolases form a schiff base intermediate between glycerone phosphate and an active site lysine residue, while the class II enzymes use a divalent cation such as zinc for catalysis. Bacteria have class II aldolases, that uses catalytic metal ion cation to polarize the carbonyl oxygen of their substrate. Zinc behaves as an electrophile and stabilizes the enolate intermediate.
Work Step by Step
Class I aldolases form a schiff base intermediate between glycerone phosphate and an active site lysine residue, while the class II enzymes use a divalent cation such as zinc for catalysis. Bacteria have class II aldolases, that uses catalytic metal ion cation to polarize the carbonyl oxygen of their substrate. Zinc behaves as an electrophile and stabilizes the enolate intermediate.
