Answer
methotrexate inhibits the synthesis of histidine and methionine by blocking the activity of DHFR, leading to a decrease in the availability of the THF cofactor required for their synthesis. Inhibition of histidine and methionine synthesis can have significant metabolic effects on the body, including impaired inflammatory and antioxidant responses.
Work Step by Step
By preventing DHFR from functioning, methotrexate prevents the production of THF by reducing the cofactor's availability. Because of this, the synthesis of histidine and methionine, which both require THF for synthesis, is also prevented.
