Answer
To find fold purification: The first sample will always have a fold purif. of 1, since it is the entire sample before purifying it; everything has been recovered. The remaining sample folds can be found by dividing each specific activity by the specific activity of the crude sample (that's a way to remember the crude sample's fold will be 1). To find percent yield: The first sample will always have a percent yield of 100%, since again, nothing has been lost. The remaining percent yields of the enzyme are found by dividing the total activity of each purification step by the crude extract's total activity and multiplying by 100 (this is how the crude extract will always be 100%).
Work Step by Step
To find fold purification: The first sample will always have a fold purif. of 1, since it is the entire sample before purifying it; everything has been recovered. The remaining sample folds can be found by dividing each specific activity by the specific activity of the crude sample (that's a way to remember the crude sample's fold will be 1). To find percent yield: The first sample will always have a percent yield of 100%, since again, nothing has been lost. The remaining percent yields of the enzyme are found by dividing the total activity of each purification step by the crude extract's total activity and multiplying by 100 (this is how the crude extract will always be 100%).
