Answer
The intact protein should be Gln-Ala-Phe-Val-Lys-Gly-Tyr-Asn-Arg-Leu-Gly Now if we observe the segments from chymotrypsin digestion, we can understand that Asn-Arg-leu-glu should be together, Val-lys-gly-Tyr should also be together and Gln-Ala-Phe should also be together. Now looking at trypsin digested parts, Gln-Ala Phe should continue with Val-Lys which again should continue as Gly-Tyr (as all four amino acid should be together observed from chymotrypsin digestion. Now again from trypsin digested parts, Gly-Tyr should continue as Asn-Arg which again should be with Leu-Gly.
Work Step by Step
The intact protein should be Gln-Ala-Phe-Val-Lys-Gly-Tyr-Asn-Arg-Leu-Gly Now if we observe the segments from chymotrypsin digestion, we can understand that Asn-Arg-leu-glu should be together, Val-lys-gly-Tyr should also be together and Gln-Ala-Phe should also be together. Now looking at trypsin digested parts, Gln-Ala Phe should continue with Val-Lys which again should continue as Gly-Tyr (as all four amino acid should be together observed from chymotrypsin digestion. Now again from trypsin digested parts, Gly-Tyr should continue as Asn-Arg which again should be with Leu-Gly.
