Fundamentals of Biochemistry: Life at the Molecular Level 5th Edition

Published by Wiley
ISBN 10: 1118918401
ISBN 13: 978-1-11891-840-1

Chapter 5 - Proteins: Primary Structure - Exercises - Page 129: 32

Answer

the sequence of the above problem is Asp met-leu-phe-met-arg-ala-tyr-gly-asn when treated with CNBr, it cleaves C terminal of amino acid methionine and the enzyme chymotrypsin cleaves at C terminal of phenylalanine/ tyrosine/ tryptophan. by corelating thses two accounts we see the 3rd fragment continues as 5th, and 4th proceeds to 1st.

Work Step by Step

the sequence of the above problem is Asp met-leu-phe-met-arg-ala-tyr-gly-asn when treated with CNBr, it cleaves C terminal of amino acid methionine and the enzyme chymotrypsin cleaves at C terminal of phenylalanine/ tyrosine/ tryptophan. by corelating thses two accounts we see the 3rd fragment continues as 5th, and 4th proceeds to 1st.
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