Answer
the sequence of the above problem is Asp met-leu-phe-met-arg-ala-tyr-gly-asn when treated with CNBr, it cleaves C terminal of amino acid methionine and the enzyme chymotrypsin cleaves at C terminal of phenylalanine/ tyrosine/ tryptophan. by corelating thses two accounts we see the 3rd fragment continues as 5th, and 4th proceeds to 1st.
Work Step by Step
the sequence of the above problem is Asp met-leu-phe-met-arg-ala-tyr-gly-asn when treated with CNBr, it cleaves C terminal of amino acid methionine and the enzyme chymotrypsin cleaves at C terminal of phenylalanine/ tyrosine/ tryptophan. by corelating thses two accounts we see the 3rd fragment continues as 5th, and 4th proceeds to 1st.