Answer
Site- Directed Mutagenesis (SDM) are based on a substitutioin of single base, which lead to change one A.A. In this replacement of one of the simillar Amino acid in overall size but differ in its chemical. Disturbance in Chemical nature of Peptide chain that lead more disturbance in structure of protein. From the above given options.
Lys replaced by Asp or Arg would expect to disrupt protein structure the most. In case of remaining options Val can not replaced by Ala or Phe. Gln can not replaced by Glu or Asn, Pro cannot replaced by His or Gly.
Work Step by Step
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