Chapter 3 - Tools of the Laboratory - Critical Thinking Questions - Bloom's Levels 3, 4, and 5: Apply, Analyze, and Evaluate - Page 78: 1

The five techniques used to manipulate, grow, examine and characterize microorganisms in laboratory (also called 5 I's) are as follows: 1. Inoculation: In this step sample is added to a sterile medium (culture) 2. Incubation: In this step optimal environment is provided for growth 3. Isolation: Strain of microbe under study is isolated from mixed population of other microbes 4. Inspection: In this step sample is observed and analyzed 5. Identification: In this step specie of the microbe under study is identified using biochemical tests.

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