Answer
Three isolation techniques are as follows:
1. Loop dilution: In this technique an inoculating loop is dipped into sample and then dipped into a series of agar with the idea that the agar in which it is last dipped will have least number of microbial colonies.
2. Streak Plating: An inoculating loop is dipped into sample and then spread across a plate making streaks. After that loop is sterilized and then touched to already streaked plate and again make streaks on another plate. This process is repeated three times with the idea that colonies of single type of bacteria are formed on last streak.
3. Spread Plating: In this method a drop of sample is placed on a plate and then spread across to make streaks to produce separate colonies.
Streak plating method is best to isolate a single type of bacterium and is most widely used in laboratories.
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