Answer
Higher salt concentration would break the weak interactions and proteins unfold hence become less globular therefore will tend to have higher sedimentation coefficients in those conditions. Sedimentation rate does not depend only on the mass or volume of a particle, and when two particles bind together there is inevitably a loss of surface area. Thus when measured separately they will have svedberg values that may not add up to that of the bound particle.
Work Step by Step
Higher salt concentration would break the weak interactions and proteins unfold hence become less globular therefore will tend to have higher sedimentation coefficients in those conditions. Sedimentation rate does not depend only on the mass or volume of a particle, and when two particles bind together there is inevitably a loss of surface area. Thus when measured separately they will have svedberg values that may not add up to that of the bound particle.
