Answer
When gel filtration was performed, the protein behaved as if it is a larger protein, which gave the impression that the protein has an elongated shape. SDS PAGE is a more accurate method for mass determination as the mobility of a denatured SDS coated protein depends only on its size. Therefore the preferred method for mass determination is SDS PAGE as it determines the mass on the basis of size in comparision to gel fltration in which the shape of the protein is ued to determine its size.
Work Step by Step
When gel filtration was performed, the protein behaved as if it is a larger protein, which gave the impression that the protein has an elongated shape. SDS PAGE is a more accurate method for mass determination as the mobility of a denatured SDS coated protein depends only on its size. Therefore the preferred method for mass determination is SDS PAGE as it determines the mass on the basis of size in comparision to gel fltration in which the shape of the protein is ued to determine its size.
