Fundamentals of Biochemistry: Life at the Molecular Level 5th Edition

Published by Wiley
ISBN 10: 1118918401
ISBN 13: 978-1-11891-840-1

Chapter 5 - Proteins: Primary Structure - Exercises - Page 127: 14

Answer

Ans 1-Glycine Ans 2- Thr Ans 3- None In wheat germ N termianl is acetylated Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide. it involves the sequential cleavage of amino acids from the N terminal end of a protein, and identification of individual amino acids using microbore HPLC. A single cycle will identify a single amino acid, and sequential cleavage will determine the amino acid sequence.

Work Step by Step

Ans 1-Glycine Ans 2- Thr Ans 3- None In wheat germ N termianl is acetylated Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide. it involves the sequential cleavage of amino acids from the N terminal end of a protein, and identification of individual amino acids using microbore HPLC. A single cycle will identify a single amino acid, and sequential cleavage will determine the amino acid sequence.
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