Answer
Now, positions where only one or two substitutions are observed can be considered to tolerate conservative substitution. For example, in position 1, all the organisms have residue D except for alligator which has residue K. Hence, there is only one substitution in position 1. Similarly, in position 8, all the organisms have residue E except for Tuna that has residue L. Hence, again there is only one substitution in position 8. Now, in position 10, all the organisms have residue L except for alligator and turtle where the residue is I. Hence, it is also able to tolerate conservative substitution with only two amino acid residues being found in all organisms at that position. Similarly, in position 5, all organisms have the residue H except for tuna and carp where the residues are M and T respectively. Hence, there are two substitutions, which can be considered as being able to tolerate conservative substitution. Hence, positions that appear to tolerate conservative substitutions are,1,5,8,10
Work Step by Step
Now, positions where only one or two substitutions are observed can be considered to tolerate conservative substitution. For example, in position 1, all the organisms have residue D except for alligator which has residue K. Hence, there is only one substitution in position 1. Similarly, in position 8, all the organisms have residue E except for Tuna that has residue L. Hence, again there is only one substitution in position 8. Now, in position 10, all the organisms have residue L except for alligator and turtle where the residue is I. Hence, it is also able to tolerate conservative substitution with only two amino acid residues being found in all organisms at that position. Similarly, in position 5, all organisms have the residue H except for tuna and carp where the residues are M and T respectively. Hence, there are two substitutions, which can be considered as being able to tolerate conservative substitution. Hence, positions that appear to tolerate conservative substitutions are,1,5,8,10
