Fundamentals of Biochemistry: Life at the Molecular Level 5th Edition

Published by Wiley
ISBN 10: 1118918401
ISBN 13: 978-1-11891-840-1

Chapter 5 - Proteins: Primary Structure - Exercises - Page 127: 13

Answer

selective adsorption can be used as a technique for the separation of the three proteins. As it can be observed the 2nd and 3rd protein have cysteine and tyrosine in them which selectively goes oxidation under a oxidative stress condition, so having an adsorption matrix that could facilitate the formation of oxidative bonds can separate the two proteins out of the mixture leaving only protein 1. Thus it could be obatined in the a pure form.

Work Step by Step

selective adsorption can be used as a technique for the separation of the three proteins. As it can be observed the 2nd and 3rd protein have cysteine and tyrosine in them which selectively goes oxidation under a oxidative stress condition, so having an adsorption matrix that could facilitate the formation of oxidative bonds can separate the two proteins out of the mixture leaving only protein 1. Thus it could be obatined in the a pure form.
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