Fundamentals of Biochemistry: Life at the Molecular Level 5th Edition

Published by Wiley
ISBN 10: 1118918401
ISBN 13: 978-1-11891-840-1

Chapter 5 - Proteins: Primary Structure - Exercises - Page 127: 3

Answer

Peptide B has greater absorbance at 280 nm. This is because peptide B contains tryptophan amino acid which due to its aromatic structure absorbs UV radiation intensely at 280 nm. Both tyrosine and tryptophan absorbs strongly at 280 nm while phenylalanine absorbs less intensely at 280 nm.

Work Step by Step

Peptide B has greater absorbance at 280 nm. This is because peptide B contains tryptophan amino acid which due to its aromatic structure absorbs UV radiation intensely at 280 nm. Both tyrosine and tryptophan absorbs strongly at 280 nm while phenylalanine absorbs less intensely at 280 nm.
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