Fundamentals of Biochemistry: Life at the Molecular Level 5th Edition

Published by Wiley
ISBN 10: 1118918401
ISBN 13: 978-1-11891-840-1

Chapter 11 - Enzymatic Catalysis - Exercises - Page 359: 15

Answer

Lysozyme cleaves between the 4th and 5th sugar and will preferably bind (NAG), and distort the conformation of the 4th sugar (D) accelerates the reaction. Moreover, since the lysozyme active site can bind at lease six monosaccharide units, (NAG), would be more tightly bound to the enzyme than (NAG) 4, and this additional binding free energy would be applied to distorting the D ring to its half-chair conformation, thereby facilitating the reaction.

Work Step by Step

Lysozyme cleaves between the 4th and 5th sugar and will preferably bind (NAG), and distort the conformation of the 4th sugar (D) accelerates the reaction. Moreover, since the lysozyme active site can bind at lease six monosaccharide units, (NAG), would be more tightly bound to the enzyme than (NAG) 4, and this additional binding free energy would be applied to distorting the D ring to its half-chair conformation, thereby facilitating the reaction.
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