Answer
The enzyme lysozyme degrades the bacterial cell wall. It hydrolyzes glycosidic bond from N-acetylmuramic (NAM) acid to N-acetylglucosamine (NAG). It converts acetal group into hemiacetal group. Glu 35 and Asp 52 are the catalytic residues of lysozymes. This enzymatic reaction should include an acid catalyst and a stabilization of transition state of oxonium ion (to convert from acetal to hemiacetal).
Steps involves
* General acid catalysis (oxonium ion formation)
* Covalent catalysis
* Covalent intermediate
* Water binding and
* Genereal base catalysis. Mutation of Glu 35 and Asp 52 to Glu is responsible for low catalytic activity of the enzyme and decreased binding property of the enzyme to substrates.
Work Step by Step
The enzyme lysozyme degrades the bacterial cell wall. It hydrolyzes glycosidic bond from N-acetylmuramic (NAM) acid to N-acetylglucosamine (NAG). It converts acetal group into hemiacetal group. Glu 35 and Asp 52 are the catalytic residues of lysozymes. This enzymatic reaction should include an acid catalyst and a stabilization of transition state of oxonium ion (to convert from acetal to hemiacetal).
Steps involves
* General acid catalysis (oxonium ion formation)
* Covalent catalysis
* Covalent intermediate
* Water binding and
* Genereal base catalysis. Mutation of Glu 35 and Asp 52 to Glu is responsible for low catalytic activity of the enzyme and decreased binding property of the enzyme to substrates.
